Allergen Data Collection: Peanut (Arachis hypogaea)
Internet Symposium on Food Allergens 2(2): 87-122 (2000) [http://www.food-allergens.de

7.5 Properties of Profilin (Ara h 5)

7.5.1 Molecular Biological Properties
 
Profilin References
Allergen Nomenclature  Ara h 5 (1) Larsen & Lowenstein 1999
Molecular Mass
Calculated 14.0 kDa (1)
(1) Kleber-Janke et al. 1999
Isoelectric Point  Calculated pI 4.6 (1)
(1) Kleber-Janke et al. 1999
Amino Acid Sequence, mRNA, and cDNA
Ara h 5  
SWISS-PROT:  
GenBank: AF059616
PIR:  
Amino acids 131 residues (1)
mRNA 743 bp
cDNA 743 bp (1)
Gene  
(1) Kleber-Janke et al. 1999
recombinant Protein
cDNA expression library:
Phage surface display of functional cDNA expression products, selection of cDNA clones on microtiter wells coated with serum IgE from peanut sensitive patients (pJuFo cloning system) (1)

Expression in Escherichia coli:
Subcloning into pDS56/RBSII vector and expression of recombinant Ara h 5 in E.coli M15 cells (1)

(1) Kleber-Janke et al. 1999
Sequence Homology
Profilin from soybean (Gly m 3): aa 83% identity (1)
(1) Kleber-Janke et al. 1999

7.5.2 Allergenic Properties
 
Profilin References
Frequency of Sensitization
IgE-binding to Ara h 5 in 13% of patients (1)
(1) see 7.1 Sensitization to Peanut Allergens



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