Allergen Data Collection: Peanut (Arachis hypogaea)
Internet Symposium on Food Allergens 2(2): 87-122 (2000) [http://www.food-allergens.de

7.6 Properties of Conglutin-homologue Protein (Ara h 6)

7.6.1 Molecular Biological Properties
 
Conglutin-homologue Protein References
Allergen Nomenclature  Ara h 6 (1) Larsen & Lowenstein 1999
Molecular Mass
Calculated 14.5 kDa (1)
(1) Kleber-Janke et al. 1999
Isoelectric Point  Calculated pI 5.2 (1)
(1) Kleber-Janke et al. 1999
Amino Acid Sequence, mRNA, and cDNA
Ara h 6  
SWISS-PROT:  
GenBank: AF092846
PIR:  
Amino acids 124 residues (1)
mRNA  
cDNA 627 bp (1)
Gene  
(1) Kleber-Janke et al. 1999
Recombinant Protein
cDNA expression library:
Phage surface display of functional cDNA expression products, selection of cDNA clones on microtiter wells coated with serum IgE from peanut sensitive patients (pJuFo cloning system) (1)

Expression in Escherichia coli:
Subcloning into pDS56/RBSII vector and expression of recombinant Ara h 6 in E.coli M15 cells (1)

(1) Kleber-Janke et al. 1999
Sequence Homology
Ara h 2 peanut allergen: aa 59% identity (1)
Ara h 7 peanut allergen: aa 35% identity (1)
Conglutin (2S-storage protein) from lupine: aa 39% identity (1)
(1) Kleber-Janke et al. 1999

7.6.2 Allergenic Properties
 
Conglutin-homologue Protein References
Frequency of Sensitization
IgE-binding to Ara h 6 in 38% of patients (1)
(1) see 7.1 Sensitization to Peanut Allergens



copyright © 2000 by matthias besler - ONLINE PUBLISHER
home: www.food-allergens.de