4.5.1 Molecularbiological Properties
| Lysozyme (LY) | References |
| Allergen Nomenclature Gal d 4 | (1) King et al. 1994 |
| Molecular Mass Mr 14.3 kDa | (1) Awade et al. 1994 |
|
Isoelectric
Point pI 11
|
(1) Holen & Elsayed 1990 |
| Amino Acid Sequence
SWISS-PROT: P01012 129 residues (1, 2) |
(1) Blake et al. 1965
(2) Imoto et al. 1981 |
| cDNA Sequence
EMBL: J00895 3.9 kb (1) |
(1) Baldacci et
al. 1979
|
| mRNA Sequence
Sequence (1) |
(1) Jung et al. 1980 |
| recombinant LY
expression in Aspergillus niger (1, 8) expression in Escherichia coli (5, 14) expression of LY mutants in Escherichia coli (7, 9) expression in Lactococcus lactis (4) expression of LY mutants in Saccharomyces cerevisiae (2, 3, 7, 11, 13) expression of glycosylated mutants in yeast (6, 15) and tabacco (15) phage display of LY (12) |
(1) Archer et al.
1990
(2) Inoue et al. 1992a (3) Inoue et al. 1992a (4) van de Guchte et al. 1992 (5) Fischer et al. 1993 (6) Nakamura et al. 1993 (7) Ueda et al. 1993 (8) Jeenes et al. 1994 (9) Tachibana et al. 1994 (10) Tsumoto et al. 1995 (11) Hashimoto et al. 1996 (12) Maenaka et al. 1996 (13) Ueda et al. 1996 (14) De Bernardez Clark et al. 1998 (15) Kato et al. 1998 |
| 3D-Structure
X-ray of LY (1) NMR study comparison with X-ray data (2) NMR of LY (4) 15N-labeled LY (NMR studies) (3) NMR studies of oxidized, reduced and, denatured LY (9) antibody-LY complexes (3, review: 8) antibody-LY mutant complex (6) LY mutants (X-ray) (7) murine MHC II - LY 50-62 complex (10) molecular modeling of murine MHC II - LY 52-61 complex (11) |
(1) Blake et al. 1965
(2) Redfield et al. 1988 (3) Fischmann et al. 1991 (4) Bartik et al. 1993 (5) Buck et al. 1995a , 1995b (6) Chacko et al. 1995 (7) Shih et al. 1995 (8) Bentley 1996 (9) Schwalbe et al. 1997 (10) Fremont et al. 1998 (11) Weber et al. 1998 |
| Posttranslational Modifications
Disulfide bonds: 4 disulfide bonds: 24-145, 48-133, 82-98, 94-112 (1) Glycosylation of LY:
|
(1) Haeffner-Gormley
et al. 1985
(2) Trudel & Asselin 1995 |
| Biological Function
bacteriolytic function: muramidase and chitinase activity 1,4-beta-N-Acetyl-muramidase C (EC 3.2.1.17) active sites: 35 / 52, substrate binding: 101 (review: 1) |
(1) Kirby 1987 |
4.5.2 Allergenic Properties
| Lysozyme (LY) | References |
| Immunoglobulines
IgE-binding studies (1) |
(1) see 4.1 Sensitization to Egg White Allergens |
| PBMC / T-Cell proliferation
lymphocyte transformation test (LTT) LY (1, 3) TCL Proliferation induced by LY (2) |
(1) Pichler &
Campi 1993
(2) Holen & Elsayed 1996 (3) Nyfeler & Pichler 1997 |