5.3.1 Molecular Biological Properties
 

Major Pollen Allergen	References
Allergen Nomenclature Ory s 1	(1) Larsen & Lowenstein 1999
Isoallergens and Variants 2 gene family as indicated by DNA gel blot analysis (1)	(1) Xu et al. 1995
Molecular Mass  35 kDa (SDS-PAGE), 26 kDa (calculated) (1)	(1) Xu et al. 1995
Isoelectric Point
Amino Acid Sequence,mRNA, and cDNA Protein Ory s 1 SWISS-PROT: Q40638 GenBank:  U31771 PIR: T03303 Amino Acids 240 mRNA precursor 1168 bp cDNA precursor	(1)  Xu et al. 1995
cDNA library / recombinant Protein cDNA library: cDNA clones from mature rice anther, screened with Cyn d 1 specific Ab  (1)	(1) Xu et al. 1995
Posttranslational Modifications Glycosylation: N-Glycosylation motif (N-X-(S/T) near N-terminus (1)	(1) Xu et al. 1995
Biological Function unknown
Sequence Homology Lol p 1 from rye grass and Cyn d 1 from bermuda grass: aa sequence identity 66% and 63% (1)	(1) Xu et al. 1995
Localization Rice pollen: Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues (1)	(1) Xu et al. 1995

 5.3.2 Allergenic Properties
 

Major Pollen Allergen	References
Frequency of Sensitization IgE-binding to Ory s 1 from pooled serum of rye grass pollen allergic patients (1)	(1) Xu et al. 1995
Cross Sensitization IgE-binding to Ory s 1 observed by serum from grass pollen allergic patients from Melbourne (Australia), where no rice aeroallergens exist. Thus Ory s 1 is unlikely to be the primary sensitzing antigen in this case (1)	(1) Xu et al. 1995
B-Cell Epitopes IgE binding sites located on (the precursor sequence): aa 235-263 (C-terminal peptide) (1)	(1) Xu et al. 1995
recombinant Protein Recombinant Ory s 1 completely abolished IgE- binding to native Ory s 1 (immunoblot inhibition) (1)	(1) Xu et al. 1995